Key result
Novel monoclonal antibodies effectively neutralize Brazilian Avian Metapneumovirus and inhibit cellular fusion in vitro.
Five monoclonal antibodies against the Brazilian isolate of Avian Metapneumovirus were successfully created and demonstrated neutralizing activity, providing a tool for specific diagnosis and serological studies.
May aid Brazilian avian metapneumovirus serodiagnosis; leaves open in vivo utility and field translation.
Avian Metapneumovirus (aMPV), also called Turkey Rhinotracheitis Virus (TRTV), is an upper respiratory tract infection of turkeys, chickens and other avian species. Five monoclonal antibodies (MAbs) were created against the Brazilian isolate (SHS-BR-121) of aMPV, MAbs 1A5B8; 1C1C4; 2C2E9 and 2A4C3 of IgG1 and MAb 1C1F8 of IgG2a. Four Mabs (1A5B8; 1C1C4; 2C2E9 and 2A4C3) showed neutralizing activity and three (1A5B8; 1C1C4 and 2A4C3) inhibited cellular fusion in vitro. These MAbs were used to investigate antigenic relationship among three strains (SHS-BR-121, STG 854/88 and TRT 1439/91) of aMPV subtypes A and B using cross-neutralization test. The results confirm that the monoclonal antibodies described can be used as a valuable tool in the epizootiological and serological studies, and also for the specific diagnosis of the subtypes in the infection for Avian Metapneumovirus.
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Coswig et al. (2007) studied Avian Metapneumovirus (aMPV). Monoclonal antibodies against aMPV (SHS-BR-121) was evaluated on Neutralizing activity and cellular fusion inhibition in vitro. Five monoclonal antibodies were created against the Brazilian isolate of Avian Metapneumovirus, with four showing neutralizing activity and three inhibiting cellular fusion in vitro.
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