Key result
Six monoclonal antibodies against avian metapneumovirus subtype C were produced and characterized, all reacting with the nucleocapsid protein without neutralizing infectivity.
The developed monoclonal antibodies against the N protein of aMPV subtype C provide new tools for investigating infection, pathogenesis, and diagnosis of aMPV disease.
Monoclonal antibodies (MAbs) were prepared against avian metapneumovirus (aMPV) subtype C (aMPV/ Minnesota/turkey/1a/97). Six MAbs were selected based on enzyme-linked immunosorbent assay activities and characterized by isotyping, neutralization test, western blot analysis, and immunohistochemistry assay. The results showed that three MAbs (3E, 9D, and 12C) belonged to the IgG1 subclass, whereas the other three (5D, 8E, and 16E) were of the IgG2a subclass. None of the six MAbs neutralized aMPV infectivity at a detectable level, but all reacted with both denatured and nondenatured forms of the nucleocapsid (N) protein of aMPV, suggesting that these MAbs may recognize structurally independent epitopes of the N protein. These MAbs provide new tools and methods for investigating aMPV infection and pathogenesis, as well as diagnosis of aMPV disease.
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Yu et al. (2006) studied Avian metapneumovirus (aMPV) subtype C. Monoclonal antibodies against aMPV subtype C was evaluated on Characterization of MAbs (isotyping, neutralization, western blot, immunohistochemistry). Six monoclonal antibodies against avian metapneumovirus subtype C were produced and characterized, all reacting with the nucleocapsid protein without neutralizing infectivity.
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