Key result
Six avian reovirus monoclonal antibodies target outer capsid proteins and demonstrate varying neutralizing activity.
Monoclonal antibodies against avian reovirus outer capsid proteins demonstrate varying neutralizing activities depending on their specific protein targets.
Hypothesis-generating for epitope-targeted mAb or vaccine strategies in avian reovirus; requires validation before any application.
Monoclonal antibodies (MAbs) were produced against a vaccinal S1133 strain of avian reovirus. Characterization of six MAbs in Western blotting, radioimmunoprecipitation and gold immunoelectron microscopy revealed that the MAbs were specific to the outer capsid proteins, mu2/mu2c, sigma2 and sigma3. Two of three MAbs, directed against sigma2 protein, neutralized the virus infectivity in a broadly specific manner, whereas the third had no neutralizing activity. The only MAb which reacted with sigma3 protein showed a type-specific neutralizing activity. Two MAbs recognizing the mu2/mu2c proteins failed to neutralize the virus infectivity.
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Shapouri et al. (1996) studied Avian reovirus. Monoclonal antibodies against avian reovirus strain S1133 was evaluated on Specificity and neutralizing activity of monoclonal antibodies. Six monoclonal antibodies against avian reovirus strain S1133 were specific to outer capsid proteins mu2/mu2c, sigma2, and sigma3, demonstrating varying degrees of neutralizing activity.
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