Key result
Co-delivery of an FMDV DNA vaccine with bovine IFN-alpha protected 25% (1 of 4) of guinea pigs from FMDV challenge, though this was lower than conventional vaccine.
Why the study?
Does co-delivery of bovine IFN-alpha with a DNA vaccine improve immune response and protection against FMDV in guinea pigs?
Does co-delivery of bovine IFN-alpha with a DNA vaccine improve immune response and protection against FMDV in guinea pigs?
Co-delivery of bovine IFN-alpha enhances the immunogenicity and protective efficacy of an FMDV DNA vaccine in guinea pigs, though it remains inferior to conventional vaccines.
Limited protection in guinea pigs with IFN-α co-delivery; leaves open whether this adjuvant strategy merits further veterinary vaccine development.
In this study, we constructed recombinant plasmid pcDNA3.1/P12X3C3D including P1, 2A, 3C, 3D and part of 2B gene of FMDV and pcDNA3.1/IFN containing the gene encoding bovine IFN-alpha. We inoculated the DNA vaccine pcDNA3.1/P12X3C3D with or without pcDNA3.1/IFN to evaluate the efficiency of this DNA vaccine and the immunogenicity of DNA vaccine enhanced by the co-delivery with pcDNA3.1/IFN. After two times of vaccination with DNA vaccine, all of guinea pigs were challenged with 103 ID50 FMDV type O. Anti-FMDV antibody levels were detected by ELISA and T lymphocyte proliferation response was tested by MTT assay. The result shows that guinea pigs inoculated by pcDNA3.1/P12X3C3D alone or with pcDNA3.1/IFN generated specific antibodies and induced an FMDV-specific T lymphocyte proliferation response. FMDV challenge tests showed that one in four guinea pigs immunized by pcDNA3.1/P12X3C3D with pcDNA3.1/IFN was protected from the FMDV serotype O infection. This result indicated that the efficiency of the DNA vaccine was enhanced by co-delivery with pcDNA3.1/IFN. However, the protection rate was considerably lower than that immunized with conventional FMD vaccine.
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Guo et al. (2004) studied Foot-and-Mouth Disease Virus (FMDV) infection. pcDNA3.1/P12X3C3D with pcDNA3.1/IFN (DNA vaccine + bovine IFN-alpha) vs. pcDNA3.1/P12X3C3D alone was evaluated on Protection from FMDV serotype O infection. Co-delivery of an FMDV DNA vaccine with bovine IFN-alpha protected 25% (1 of 4) of guinea pigs from FMDV challenge, though this was lower than conventional vaccine.
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