Galectin 3 and non-classical monocytes of blood as myocardial remodeling factors at ischemic cardiomyopathy

Fig. 1. The subpopulation composition of peripheral blood monocytes in (A) healthy donors and CHD patients: (B) without ICMP and (C) with ICMP. Distribution of monocytes into subpopulations in patient with ICMP: R1 – classical (CD14++CD16−) monocytes, R2 – intermediate (CD14++CD16+) monocytes, R3 – non-classical (CD14+CD16++) monocytes, R4 – transitional (CD14+CD16−) monocytes.

Fig. 2. The content of macrophages in biopsy samples of myocardium from the patients with ICMP. (A) The myocardium of the left ventricular aneurysm in a patient with ICMP. (B) The myocardium of the right atrium appendage in a patient with ICMP. Note. CD68+ cells (brown stain), hematoxylin counterstain, ×400.

Fig. 3. The relationship between the concentration of heart remodeling mediators in peripheral and sinus blood in CHD patients, with and without ICMP. In pooled sample of CHD patients, with and without ICMP, a non-linear relationship between concentrations of galectin-3 in peripheral and sinus blood was demonstrated. Spearman's rank correlation coefficient (r) and correlation ratio (ɳ) calculation were used.

Fig. 4. An integral map of relationships between the concentration of myocardial remodeling mediators in peripheral and sinus blood and the subpopulation composition of peripheral blood monocytes in CHD patients: (A) without ICMP and (B) with ICMP. Note. rs – Spearman's rank correlation coefficient, p – the level of statistical significance.

Fig. 5. The role of cardiac remodeling factors and subpopulation of blood monocytes in the pathogenesis of ischemic cardiomyopathy. Gal-3 – galectin 3, ECM – extracellular matrix, CMC – cardiomyocytes.