Key result
A simple procedure using hot xylene dewaxing followed by acid phenol extraction and RNA PCR successfully detected short RNA target sequences from various viruses in single wax tissue sections.
Population
Single sections of wax-embedded tissue from various tissues not deliberately prepared for this purpose
Authors
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May enable retrospective viral RNA studies in archived tissues; leaves open diagnostic adoption pending validation.
This simple technique allows for the detection of specific RNA species and viruses in stored histological tissue samples, enabling the study of gene expression and viral replication distribution.
Woodall et al. (1993) studied RNA viruses in wax-embedded tissue. Hot xylene dewaxing followed by acid phenol extraction of RNA and RNA PCR was evaluated on Detection of short RNA target sequences (130-420 bases). A simple procedure using hot xylene dewaxing followed by acid phenol extraction and RNA PCR successfully detected short RNA target sequences from various viruses in single wax tissue sections.
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