Key result
Double-nested PCR with magnetic separation detects infectious pancreatic necrosis virus down to ~0.8 pg RNA.
Why the study?
A sensitive and specific method was needed to detect infectious pancreatic necrosis virus from salmonid fish using PCR and magnetic separation techniques.
A double-nested PCR assay combined with magnetic separation successfully and sensitively detects infectious pancreatic necrosis virus RNA from salmonid fish.
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May enable sensitive IPNV detection in salmonid aquaculture; leaves open routine diagnostic adoption pending field validation.
Rimstad et al. (1990) studied Infectious pancreatic necrosis virus in salmonid fish. Double-nested polymerase chain reaction (PCR) assay followed by magnetic separation was evaluated on Detection of double-stranded RNA virus. A double-nested PCR assay followed by magnetic separation successfully detected infectious pancreatic necrosis virus with a sensitivity of approximately 0.8 pg of template double-stranded RNA.