What question did this study set out to answer?

Characterize the enzymatic properties, kinetics, and regulation of angiotensin-converting enzyme (ACE) within intact microvessels isolated from rabbit cerebral cortex.

March 1, 1981HypertensionOpen Access

Properties of angiotensin-converting enzyme in intact cerebral microvessels.

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Authors

PBPeter BrecherBoston University ATAnn TercyakBoston University ACAram V. ChobanianSumy State A.S.Makarenko Pedagogical University

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Implication

Experimental study demonstrates resilient angiotensin-converting enzyme activity in rabbit cerebral microvessels, highlighting its stability on intact cerebrovascular endothelium.

Key Points

Characterize the enzymatic properties, kinetics, and regulation of angiotensin-converting enzyme (ACE) within intact microvessels isolated from rabbit cerebral cortex.
Isolated intact microvessels from rabbit cerebral cortex and maintained them in physiological salt solution at pH 7.4.
Assessed ACE activity by measuring the degradation of hippuryl-histidyl-leucine and angiotensin I under varying substrate and chloride concentrations.
Evaluated enzyme response to captopril inhibition (10⁻⁶ M), hyperosmolar urea pretreatment, and hypoxic or anoxic conditions.
ACE activity in intact microvessels depended on substrate and chloride ion concentrations and was inhibited by captopril, though angiotensin I degradation continued even with 10⁻⁶ M captopril.
Pretreatment with hyperosmolar urea released lactic dehydrogenase and lysosomal enzymes without altering ACE activity or causing ACE release from microvessels.
Enzyme activity remained unaffected by hypoxic or anoxic incubation, whereas captopril pretreatment produced a reversible lag in subsequent substrate cleavage.

Cite This Study

Brecher et al. (1981) studied this question.

synapsesocial.com/papers/6aa36f1ce0a56e090ff42510 https://doi.org/10.1161/01.hyp.3.2.198

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Implication

Experimental study demonstrates resilient angiotensin-converting enzyme activity in rabbit cerebral microvessels, highlighting its stability on intact cerebrovascular endothelium.

Properties of angiotensin-converting enzyme in intact cerebral microvessels.

Discussion

Member takes

Implication

Key Points

Cite This Study

Properties of angiotensin-converting enzyme in intact cerebral microvessels.

Discussion

Member takes

Implication

Key Points

Cite This Study

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