Key result
A reliable plaque assay using HeLa cell bottle cultures under starch gel and Ionagar overlay media successfully produced plaques for 60 rhinovirus strains representing 55 distinct serotypes.
Population
60 rhinovirus strains representing 55 distinct serotypes and one subtype in HeLa cell bottle cultures
Design
Preclinical
Authors
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Supports expanded rhinovirus research but warrants no clinical practice changes; leaves open therapeutic applications.
Describes a reliable plaque assay methodology for investigating rhinovirus plaque formation.
Conant et al. (1968) studied this question. A reliable plaque assay using HeLa cell bottle cultures under starch gel and Ionagar overlay media successfully produced plaques for 60 rhinovirus strains representing 55 distinct serotypes.
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