Key Points
- To evaluate antigenic relationships and cross-neutralization patterns among 90 classified rhinovirus serotypes using potent rabbit antisera.
- Generated potent antisera in rabbits against all 90 recognized rhinovirus serotypes using standard immunization protocols.
- Performed systematic neutralization tests to identify cross-relationships and compared findings with reference guinea pig antisera from the National Institutes of Health.
- Assayed original rhinovirus isolates against rabbit antisera directed against antigenically related serotypes.
- Extensive antigenic variation was observed among reciprocally related serotype pairs (12:78 and 36:58), serotypes 41 and 13, and serotype 67, which demonstrated relatedness to both serotypes 9 and 32.
- Cross-reactivity patterns enabled the classification of 50 rhinovirus serotypes into 16 distinct antigenic groups.
Structured PICO
PPopulation90 classified rhinovirus serotypes and original rhinovirus isolates
IInterventionNeutralization tests using rabbit antisera
CComparatorNational Institutes of Health reference guinea pig antisera
OOutcomeAntigenic cross-relationships and variations
Demonstrates significant antigenic cross-relationships among rhinovirus serotypes, allowing 50 types to be classified into 16 groups.