Key Points
- To assess the extent of cross-neutralization among 37 distinct rhinovirus serotypes using potent monotypic rabbit antisera.
- Generated monotypic antisera in rabbits against 37 individual rhinovirus (RV) serotypes.
- Assayed each monotypic antiserum against 36 heterologous RV serotypes in virus neutralization tests.
- Measured neutralizing antibody titers and neutralization rate constants across serial serum collections to assess antibody response kinetics.
- Reciprocal neutralization was detected specifically between RV types 2 and 49, as well as between RV types 13 and 41.
- Five additional monotypic antisera exhibited one-way neutralization against a single heterologous rhinovirus serotype.
- Neutralizing antibody titers and neutralization rate constants followed a parallel time pattern for both immunizing and cross-reacting serotypes without threatening false serotype identification.
Structured PICO
PPopulationRabbits immunized to produce monotypic antisera against 37 rhinovirus (RV) types
IInterventionTesting for cross-neutralization against 36 heterologous rhinovirus types
OOutcomeReciprocal neutralization and neutralizing antibody titers against heterologous RV serotypessurrogate
Monotypic rabbit antisera to 37 rhinovirus types demonstrate specific cross-neutralization patterns that do not interfere with accurate serotype identification.