November 26, 2018Clinical EpigeneticsOpen Access

Differential open chromatin profile and transcriptomic signature define depot-specific human subcutaneous preadipocytes: primary outcomes

Q: What is the clinical evidence from this study?

Study design: Observational. Population: Obesity (n=6). Intervention: Abdominal subcutaneous preadipocytes vs. Gluteofemoral subcutaneous preadipocytes. Primary outcome: Differential gene expression and open chromatin regions.

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Key result

Abdominal preadipocytes exhibit distinct epigenetic signatures with ~10-fold more accessible chromatin regions than gluteofemoral cells.

Why the study?

The molecular basis for depot-specific differences in gluteofemoral compared with abdominal subcutaneous adipocyte function is poorly understood.

Population

Premenopausal apple-shaped women

Comparison

Abdominal vs gluteofemoral subcutaneous adipose tissue preadipocytes

Authors

ADAdeline DivouxAdventHealth Orlando KSKatalin SándorStanford University DBDóra BojcsukUniversity of Debrecen

Discussion

Member takes

Implication

Regional preadipocyte epigenetic differences may underlie depot-specific cardiometabolic risks; leaves open whether chromatin signatures drive clinical outcomes.

Study Design

Type

Observational (n=6)

Multicenter

Structured PICO

Population

6 healthy, premenopausal, obese women aged 20-40 years provided abdominal and gluteofemoral subcutaneous adipose tissue biopsies for preadipocyte isolation and epigenetic profiling.

Exposure

Isolation and culture of preadipocytes from abdominal subcutaneous adipose tissue

Comparator

Isolation and culture of preadipocytes from gluteofemoral subcutaneous adipose tissue

Outcome

Depot-specific gene expression patterns and differential epigenetic chromatin signatures (assessed by ATAC-seq, RNA-seq, and ChIP-qPCR)surrogate

Abdominal and gluteofemoral subcutaneous preadipocytes exhibit distinct transcriptomic and open chromatin profiles, providing a molecular basis for depot-specific differences in fat distribution and cardiometabolic risk.

Limitations

Genome-wide analysis was performed on only two apple-shaped women.
Did not include women presenting with a gynoid fat pattern.
Did not address the stability of chromatin and histone modifications through the process of differentiation.

Cite This Study

Divoux et al. (2018) conducted an observational in Obesity (n=6). Abdominal subcutaneous preadipocytes vs. Gluteofemoral subcutaneous preadipocytes was evaluated on Differential gene expression and open chromatin regions. Abdominal and gluteofemoral preadipocytes exhibited distinct epigenetic and transcriptomic signatures, with 126 genes upregulated in abdominal cells and almost 10-times more abdominal-specific chromatin-accessible regions.

synapsesocial.com/papers/6aa3dc53401b0278c3c97ef7 https://doi.org/10.1186/s13148-018-0582-0

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November 26, 2018Clinical EpigeneticsOpen Access

Differential open chromatin profile and transcriptomic signature define depot-specific human subcutaneous preadipocytes: primary outcomes

View Full Paper

Ask AI

Bookmark

Key result

Abdominal preadipocytes exhibit distinct epigenetic signatures with ~10-fold more accessible chromatin regions than gluteofemoral cells.

Why the study?

The molecular basis for depot-specific differences in gluteofemoral compared with abdominal subcutaneous adipocyte function is poorly understood.

Population

Premenopausal apple-shaped women

Comparison

Abdominal vs gluteofemoral subcutaneous adipose tissue preadipocytes

Authors

ADAdeline DivouxAdventHealth Orlando KSKatalin SándorStanford University DBDóra BojcsukUniversity of Debrecen

Discussion

Member takes

Implication

Regional preadipocyte epigenetic differences may underlie depot-specific cardiometabolic risks; leaves open whether chromatin signatures drive clinical outcomes.

Study Design

Type

Observational (n=6)

Multicenter

Structured PICO

Population

6 healthy, premenopausal, obese women aged 20-40 years provided abdominal and gluteofemoral subcutaneous adipose tissue biopsies for preadipocyte isolation and epigenetic profiling.

Exposure

Isolation and culture of preadipocytes from abdominal subcutaneous adipose tissue

Comparator

Isolation and culture of preadipocytes from gluteofemoral subcutaneous adipose tissue

Outcome

Depot-specific gene expression patterns and differential epigenetic chromatin signatures (assessed by ATAC-seq, RNA-seq, and ChIP-qPCR)surrogate

Limitations

Genome-wide analysis was performed on only two apple-shaped women.
Did not include women presenting with a gynoid fat pattern.
Did not address the stability of chromatin and histone modifications through the process of differentiation.

Cite This Study

synapsesocial.com/papers/6aa3dc53401b0278c3c97ef7 https://doi.org/10.1186/s13148-018-0582-0

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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