Key result
Caffeine decreases and procaine increases Ca2+-induced Ca2+ release in fragmented sarcoplasmic reticulum.
Population
Heavy and light fractions of fragmented sarcoplasmic reticulum isolated from frog and rabbit skeletal muscle
Design
Preclinical
Authors
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Demonstrates Ca2+-induced Ca2+ release in isolated skeletal SR; leaves open relevance to human cardiac excitation-contraction coupling.
The study demonstrates that heavy fractions of fragmented sarcoplasmic reticulum from skeletal muscle exhibit Ca2+-induced Ca2+ release mechanisms similar to those observed in skinned muscle fibers.
Kirino et al. (1982) studied this question. Free Ca2+ concentration and pharmacological agents (caffeine, procaine, ruthenium red) was evaluated on Ca2+ uptake and release. Heavy fractions of fragmented sarcoplasmic reticulum exhibited Ca2+-induced Ca2+ release at free Ca2+ concentrations as low as 10(-6) M, which was decreased by caffeine and increased by procaine.
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