Key result
F6 10G receptor outperforms alternative receptors for electrochemical detection of equine arteritis virus protein.
Why the study?
The study was conducted to establish a methodology for selecting the optimal receptor to create an electrochemical impedance immunosensor for equine arteritis virus protein detection.
The F6 10G receptor was identified as the optimal receptor for an electrochemical immunosensor detecting equine arteritis virus protein.
May enable equine virus biosensors; leaves open clinical translation from animal data.
The study reports a methodology of selecting the optimal receptor to create an electrochemical immunosensor for equine arteritis virus (EAV) protein detection. The detection was based on antigen recognition by antibodies immobilized on gold electrodes. Modification steps were controlled by electrochemical impedance spectroscopy and cyclic voltammetry measurements. In order to obtain the impedance immunosensor with the best parameters, seven different receptors complementary to equine arteritis virus protein were used. In order to make the selection, a rapid screening test was carried out to check the sensor’s response to blank, extremely low and high concentrations of target EAV protein, and negative sample: M protein from Streptococcus equi and glycoprotein G from Equid alphaherpesvirus 1. F6 10G receptor showed the best performance.
No takes yet. Share an insight, caveat, or question.
Brodowski et al. (2021) studied Equine arteritis virus (EAV) protein detection. Seven different receptors complementary to EAV protein vs. Blank and negative samples (M protein from Streptococcus equi, glycoprotein G from Equid alphaherpesvirus 1) was evaluated on Sensor's response to blank, low/high concentrations of target EAV protein, and negative samples. Among seven different receptors tested for an electrochemical immunosensor for equine arteritis virus protein detection, the F6 10G receptor showed the best performance.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: