Key result
Upstream constitutive introns, suboptimal splice sites, and intron length determine TPO gene alternative splicing rates.
Population
Hep3B cell line transfected with minigenes of human thrombopoietin (TPO) genomic constructs
Design
Preclinical
Authors
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Preclinical TPO splicing data warrant clinical caution; leaves open human relevance while extending splice regulation models.
The recognition of suboptimal intra-exonic splice junctions in the TPO gene is influenced by spliceosome assembly on constitutive introns and sequence scanning primed by upstream splicing signals.
Maurizio Romano (2001) studied Thrombopoietin gene splicing. TPO gene minigenes vs. TPO cDNA minigenes was evaluated on Splicing of the 116 nt alternative intron. The final rate of the alternative intron splicing in the TPO gene is determined by the presence of upstream constitutive introns, suboptimal splice sites, and the length of the alternative intron.
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