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Restriction endonuclease analysis differentiates Marek's disease virus serotypes, strains, and cell-culture passage history.
Restriction endonuclease analysis is an effective method for differentiating Marek's disease virus serotypes, strains, and passage histories.
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Enables MDV strain differentiation in vitro; extends molecular tools for avian research but leaves open clinical translation.
The restriction endonuclease (RE) patterns of DNA from serotype 1 Marek's disease viruses (MDVs) are unique to serotype 1 viruses and can also be used to differentiate between low and high cell-culture-passaged viruses. We compared the RE patterns of DNA from seven serotype 2 and 3 MDVs before and after serial in vitro passage. Passage of four serotype 2 strains resulted in a variety of changes in the RE pattern. Individual strains within a serotype exhibited unique restriction patterns that allowed individual isolates to be differentiated. In a similar manner, the serotype 3 virus strains displayed RE pattern variations that were unique to each strain, as well as differences between low and high cell-culture passage. Our findings, together with earlier reports, suggest that the RE patterns of MDV DNA provide a simple and accurate method to: 1) differentiate between the three MDV serotypes, 2) differentiate between virus strains within a serotype, and 3) determine whether the viruses have been passaged extensively in cell culture.
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R. F. Silva (1991) studied Marek's Disease Virus (n=7). Serial in vitro passage vs. Low cell-culture passage was evaluated on Restriction endonuclease (RE) patterns of DNA. Restriction endonuclease analysis of Marek's disease virus DNA allowed differentiation between serotypes, individual strains, and low versus high cell-culture passage history.
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