Key result
New one-step rRT-PCR detects EV-D68 with 100% specificity and high sensitivity in respiratory samples.
Why the study?
Does a new rRT-PCR targeting the VP4-VP2 region accurately detect and quantify enterovirus D68 in respiratory samples?
Does a new rRT-PCR targeting the VP4-VP2 region accurately detect and quantify enterovirus D68 in respiratory samples?
A newly designed rRT-PCR assay targeting the VP4-VP2 region provides highly specific and sensitive detection and quantification of enterovirus D68 in respiratory samples.
May aid EV-D68 detection in respiratory samples; leaves open prospective clinical validation.
Causing an international outbreak of respiratory disease, Enterovirus D68 quickly entered the closed circle of emerging viral pathogens of public health significance. As rapid and accurate detection of EV-D68 is essential for an efficient clinical management, we designed and validated a new highly efficient one-step quantitative rRT-PCR specific to EV-D68 VP4-VP2 region. With 100% specificity and 95.6% sensitivity to all EV-D68 strains, this new assay can be reliably used to detect and quantify EV-D68 in respiratory samples and represents an interesting additional tool for diagnosis as it targets an original region of the genome.
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Royston et al. (2017) studied Enterovirus D68 respiratory infection. One-step quantitative rRT-PCR specific to EV-D68 VP4-VP2 region was evaluated on Specificity and sensitivity to EV-D68 strains. A new one-step quantitative rRT-PCR targeting the VP4-VP2 region demonstrated 100% specificity and 95.6% sensitivity for detecting and quantifying enterovirus D68 in respiratory samples.
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