Key result
A newly developed real-time RT-PCR assay for EV-D68 demonstrated an analytic limit of detection of 4 copies per reaction and was more sensitive than commercially available respiratory panels.
Population
Clinical specimens and viral strains (EV-D68, other enteroviruses, rhinoviruses)
Comparison
Newly developed real-time reverse transcriptase… vs Commercially available assays that detect…
Design
Preclinical
Authors
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Requires clinical validation before use; leaves open enhanced EV-D68 detection in research settings.
A newly developed RT-PCR assay provides highly sensitive and specific detection of EV-D68, which will be useful for identifying and studying outbreaks.
Wylie et al. (2015) studied Human enterovirus D68 (EV-D68). EV-D68 real-time reverse transcriptase PCR (RT-PCR) assay vs. Commercially available assays was evaluated on Analytic limit of detection and sensitivity. A newly developed real-time RT-PCR assay for EV-D68 demonstrated an analytic limit of detection of 4 copies per reaction and was more sensitive than commercially available respiratory panels.
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