Key result
A newly developed EV-D68-specific real-time RT-PCR assay demonstrated a 95% limit of detection of 13 copies/reaction and successfully detected all EV-D68 positive samples with no cross-reactivity.
Why the study?
Does a new real-time RT-PCR assay accurately detect human enterovirus 68 in respiratory samples?
Does a new real-time RT-PCR assay accurately detect human enterovirus 68 in respiratory samples?
A newly developed real-time RT-PCR assay enables rapid and specific detection of human enterovirus 68 in respiratory samples.
Answers are grounded in this paper and cite their sources.
May aid specific EV-D68 detection in respiratory samples; leaves open clinical validation before routine use.
A global reemergence of human enterovirus 68 (EV-D68) associated with severe respiratory illness occurred in 2014. We developed and validated an EV-D68-specific real-time reverse transcription-PCR (RT-PCR) for the detection of EV-D68 in respiratory samples. The rapid diagnosis of EV-D68 may contribute to better management of EV-D68 infections.
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Piralla et al. (2015) studied Human enterovirus 68 (EV-D68) infection. EV-D68-specific real-time RT-PCR assay was evaluated on Limit of detection (LOD) and assay specificity/inclusivity. A newly developed EV-D68-specific real-time RT-PCR assay demonstrated a 95% limit of detection of 13 copies/reaction and successfully detected all EV-D68 positive samples with no cross-reactivity.
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