Development of RT-qPCR for quantification of human enterovirus D68 in vitro

Figures

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  Fig. 1. shows the nucleotide sequences for the forward primer (FWD), reverse primer (REV) and the probe for the human enterovirus D68 detection.

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  Fig. 2. shows the different temperature-regulated steps during the RT-qPCR. The amplification cycle consists of a total of 40 cycles. The first step is the Revers Transcription step where RNA is converted to DNA at 50°. Followed by a Hot start for the amplification of the genetic material.

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  Fig. 3. Standard curve 1 generated from RT-qPCR assays of the dilution series of extracted RNA from EV-D68. The ten-fold dilution was done with Rnase-free water before run in duplicates. The different TCID50 values and Cq values measured are presented for the six first dilutions. Dilution number six was the lowest concentration measured, excluding the other three.

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  Fig. 4. Standard curve 2 generated from RT-qPCR assays of the dilution series of extracted RNA from EV-D68. The ten-fold dilution was done with Rnase-free water before run in duplicates. The different TCID50 values and Cq values measured are presented for the six first dilutions. Dilution number six was the lowest concentration measured, excluding the other three.