Myocardial protection of S-nitroso-L-cysteine in diabetic cardiomyopathy mice

Figures

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  Figure 1. Mouse body weight, heart weight and blood glucose levels. (A) A flowchart showing the experimental process for modeling mice. (B) Body weight of mice. (C) Heart weight of mice. (D) Heart weight to body weight ratio (HW/BW). (E) Heart rate (HR). (F) Random body glucose(RBG). (G) Fasting blood glucose levels (FBG). (H) After fasting for 16 hours, mice were given glucose solution by gavage, and the corresponding blood glucose values were measured at different time points. The corresponding curve was drawn, namely, an oral glucose tolerance test (OGTT) curve. (I) The area under the curve (AUCglucose) was quantified. * represents p < 0.05; ** represents p < 0.01; *** represents p < 0.001. The “ns” symbol stands for “no significance”.

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  Figure 2. The effect of SNO on cardiac function in diabetic mice. Indices of cardiac systolic function, (A) Left ventricular ejection fraction (EF %). (B) Fractional shortening (FS %). Indices of cardiac diastolic function, (C) Early-to-late septal annulus motion in diastole (E’/A’) and (D) Isovolumic relaxation time (IVRT). (E) HE staining, Sirius red staining, Masson trichrome staining and WGA staining of mouse hearts. Scale bar=50 mm. (F) The percentage of fibrotic area was quantified. (G) The percentage of LV collagen area was quantified. (H) Quantitative analysis of cardiac myocyte cross-sections. (I-L) Relative ANP, BNP, β-MHC and Col-1 mRNA expression normalized to GAPDH. * represents p < 0.05; ** represents p < 0.01; *** represents p < 0.001.

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  Figure 3. CSNO promoted cellular glucose uptake. (A) H9c2 cells were incubated with CSNO at different concentrations for 1 h. Cell viability was examined using a CCK8 kit. (B) The corresponding curve diagram of cell viability. (C) CSNO promoted cellular glucose uptake in a concentration-dependent manner. (D) CSNO increased insulin-dependent and noninsulin-dependent glucose uptake. * represents p < 0.05; ** represents p < 0.01; *** represents p < 0.001.

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  Figure 4. CSNO promoted GLUT4 membrane translocation and the insulin signaling pathway. (A) After the cytoplasm and cell membrane were separated, the expression of GLUT4 on membrane protein was detected. It was normalized to N-cadherin. (B) CSNO concentration-dependently activated the insulin signaling pathway in H9c2 cells. The expression level and phosphorylation level of Akt, IRS and IR were analyzed by Western blot. The protein band diagram and quantitative statistical diagram are shown. (C) CSNO time-dependently activated the insulin signaling pathway in H9c2 cells. The expression level and phosphorylation level of Akt, IRS and IR were analyzed by Western blot. The protein band diagram and quantitative statistical diagram are shown. (D) CSNO promoted activation of the insulin signaling pathway and further increased the effect of insulin on the insulin signaling pathway. The expression level and phosphorylation level of Akt, IRS and IR were analyzed by Western blot. The protein band diagram and quantitative statistical diagram are shown. * represents p < 0.05; *** represents p < 0.001.

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  Figure 5. CSNO alleviated insulin resistance. (A) Glucose uptake was detected by fluorescence analysis of 2-NBDG uptake by cells. Scale bar=100 μm. (B) The statistical graph of fluorescence intensity which was analyzed with imageJ software. Scale bar=100 μm. (C) The expression level and phosphorylation level of Akt, IRS and IR were analyzed by Western blot. (D) After the cytoplasm and cell membrane were separated, the expression of GLUT4 on membrane protein was detected. It was normalized to N-cadherin. ** represents p < 0.01; ***represents p < 0.001.

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  Figure 6. CSNO inhibited high glucose-induced excessive autophagy. (A-C) The expression levels of P62, LC3 II/I and GAPDH were analyzed by Western blot. The protein band diagram and quantitative statistical diagram are shown. (D, E) After adding the autophagy inhibitor Baf-A1,the expression of LC3 II/I and GAPDH was analyzed by Western blot. The protein band diagram and quantitative statistical diagram are shown. (F) An adenovirus expressing GFP-RFP-LC3 protein was used to detect autophagic flux. Scale bar=10 μm. (G) To quantify yellow and red LC3 dots per cell in each condition, Image-Pro Plus was used. * represents p < 0.05; ** represents p < 0.01; *** represents p < 0.001.

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  Figure 7. CSNO ameliorated high glucose-induced mitochondrial dysfunction. (A) ATP assay kits were used to determine cellular ATP levels. (B) Analysis of mitochondria stained with MitoTracker using confocal microscopy. Scale bar =10 μm. (C-E) MiNa in ImageJ was used to analyze the structural characteristics of the mitochondrial network, including individuals, mitochondrial footprint and mean network size. (F) Mitochondrial membrane potential (MMP) was determined by staining with JC1. JC-1 aggregates emit red fluorescence in healthy mitochondria with polarized inner mitochondria. As MMP dissipates, cytosolic monomers of JC-1 emit green fluorescence. Scale bar=50 μm. (G) A fluorescence ratio of red to green was used to calculate the MMP of cardiomyocytes for each group. * represents p < 0.05; ** represents p < 0.01; *** represents p < 0.001.

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  Figure 8. CSNO promoted high glucose-induced oxidative stress. (A, B) Analysis of reactive oxygen species (ROS) by dihydroethidium (DHE). Scale bar =100 μm. (C, D) The size of the cell was determined by detecting filaments of F-actin in the cell cytoskeleton using phalloidin. Scale bar=100 μm. ** represents p < 0.01; *** represents p < 0.001.