What is the clinical evidence from this study?

Study design: Other. Population: Airway hyperresponsiveness. Intervention: PKC activation (phorbol esters and thrombin). Primary outcome: Ca2+ signaling, Ca2+ sensitivity, protein phosphorylation, and contraction in smooth muscle cells.

What does this research mean for the field?

Protein kinase C activation in small airway smooth muscle induces contraction through both the generation of Ca2+ oscillations and CPI-17-associated Ca2+ sensitization. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

January 28, 2013The Journal of General PhysiologyOpen Access

Ca2+ oscillations, Ca2+ sensitization, and contraction activated by protein kinase C in small airway smooth muscle

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Key result

PKC activation in small airways triggers Ca2+ oscillations and sensitization, potentially inducing airway hyperresponsiveness.

Population

Mouse lung slices (small intrapulmonary airways)

Design

Preclinical

Authors

SMSeema MukherjeeTexas Tech University JTJacquelyn Trice PSPaurvi ShindeFred Hutch Cancer Center

Discussion

Member takes

Implication

May suggest PKC pathway for airway hyperresponsiveness therapies; leaves open translation from animal models to human disease.

Structured PICO

Population

Mouse lung slices used to study the effects of PKC activation on small intrapulmonary airway smooth muscle cells.

Intervention

PKC activation via 10 µM phorbol-12-myristate-13-acetate, 1 µM phorbol 12,13-dibutyrate, or thrombin

Outcome

Ca(2+) signaling, Ca(2+) sensitivity, protein phosphorylation, and contraction in smooth muscle cellssurrogate

PKC activation in small airways leads to Ca2+ oscillations and strong Ca2+ sensitization, providing a potential mechanism for airway hyperresponsiveness.

Cite This Study

Mukherjee et al. (2013) studied Airway hyperresponsiveness. PKC activation (phorbol esters and thrombin) was evaluated on Ca2+ signaling, Ca2+ sensitivity, protein phosphorylation, and contraction in smooth muscle cells. PKC activation in small airways leads to the generation of Ca2+ oscillations and strong Ca2+ sensitization, which may induce airway hyperresponsiveness.

synapsesocial.com/papers/6aa51d03c616a76b5feeb39d https://doi.org/10.1085/jgp.201210876

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Ca2+ oscillations, Ca2+ sensitization, and contraction activated by protein kinase C in small airway smooth muscle

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Ca2+ oscillations, Ca2+ sensitization, and contraction activated by protein kinase C in small airway smooth muscle

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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