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Freeze-drying of poliovirus and other picornaviruses using an ultrafiltration procedure and alkaline organic buffer allowed for excellent retention of infectivity even at ambient temperatures up to 37 C.
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A freeze-drying method using ultrafiltration and alkaline organic buffer successfully stabilizes enteroviruses for ambient temperature shipping.
A method was developed for freeze-dry stabilization of poliovirus type 3. An ultrafiltration procedure was used to remove salts from infected tissue culture fluid, and the virus was freeze-dried after suspension in an alkaline organic buffer. This method was further tested with other picornaviruses including poliovirus types 1 and 2, coxsackieviruses A9, A20, B2, and B5, echovirus 11, and the encephalomyocarditis virus. Freeze-dried preparations of the poliovirus could be shipped to distant laboratories at ambient temperature with excellent retention of infectivity. Data are presented showing effects of freeze-drying as well as results of exposure to temperatures to 37 C.
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Berge et al. (1971) studied Enterovirus preservation. Freeze-drying after ultrafiltration and suspension in an alkaline organic buffer was evaluated on Retention of infectivity after freeze-drying and exposure to temperatures up to 37 C. Freeze-drying of poliovirus and other picornaviruses using an ultrafiltration procedure and alkaline organic buffer allowed for excellent retention of infectivity even at ambient temperatures up to 37 C.
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