What question did this study set out to answer?

To determine the molecular characteristics and biological activity of circulating prolactin in women presenting with idiopathic hyperprolactinemia and normal menses.

August 1, 1985The Journal of Clinical Endocrinology & Metabolism

Characterization of a Large Molecular Weight Prolactin in Women with Idiopathic Hyperprolactinemia and Normal Menses*

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Authors

RJRebecca D. JacksonOregon State University JWJacobo WortsmanRosalind Franklin University of Medicine and Science WMWilliam B. MalarkeyInstitute for Behavioral Medicine

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Implication

Laboratory study reveals a 150,000-dalton prolactin with diminished bioactivity in women with idiopathic hyperprolactinemia, explaining their preserved menstrual function.

Key Points

To determine the molecular characteristics and biological activity of circulating prolactin in women presenting with idiopathic hyperprolactinemia and normal menses.
Analyzed serum from 5 women with idiopathic hyperprolactinemia and normal menses using gel filtration under native and denaturing conditions.
Evaluated the effect of disulfide bond reduction on molecular size and tested hormone behavior during secretory perturbations in one subject.
Assessed biological activity of the high molecular weight prolactin using the Nb2 rat lymphoma bioassay.
Gel filtration demonstrated a 98% to 100% predominance of a 150,000- to 160,000-dalton prolactin species, contrasting with the normal 22,000-dalton monomer.
The large prolactin was immunologically cross-reactive with monomeric prolactin and dissociated into smaller peptides following disulfide bond reduction.
Bioassay in Nb2 rat lymphoma cells demonstrated markedly diminished bioactivity of the 150,000- to 160,000-dalton form compared to the 22,000-dalton hormone.

Cite This Study

Jackson et al. (1985) studied this question.

synapsesocial.com/papers/6aa5a9af39a5015a928e412d https://doi.org/10.1210/jcem-61-2-258

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Implication

Laboratory study reveals a 150,000-dalton prolactin with diminished bioactivity in women with idiopathic hyperprolactinemia, explaining their preserved menstrual function.

Characterization of a Large Molecular Weight Prolactin in Women with Idiopathic Hyperprolactinemia and Normal Menses*

Discussion

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Implication

Key Points

Cite This Study

Characterization of a Large Molecular Weight Prolactin in Women with Idiopathic Hyperprolactinemia and Normal Menses*

Discussion

Member takes

Implication

Key Points

Cite This Study

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