Key result
Rapid UHPLC method measures doxorubicin and its metabolites in ~10 μL mouse plasma volumes.
A novel UHPLC method allows for rapid and highly sensitive quantification of doxorubicin and its metabolites in minute plasma volumes, reducing the number of animals needed for pharmacokinetic studies.
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May reduce animals needed for murine doxorubicin PK studies; leaves open clinical translation pending validation.
Sakai‐Kato et al. (2012) studied Pharmacokinetics of doxorubicin (n=3). Doxorubicin was evaluated on Area under the curve (AUC0-24h) of doxorubicin. A rapid and sensitive UHPLC method was developed to measure doxorubicin and its metabolites in small plasma volumes (10 μL), yielding an AUC0-24h of 5.9 μg h/mL in mice.
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