Key result
Purification of FA strain hemagglutinin of mouse encephalomyelitis virus was achieved by removing nonviral nitrogen via ether extraction and hemagglutination-elution, though methanol and acetone precipitation resulted in poor yields.
Acetone precipitation combined with ether extraction and erythrocyte adsorption/elution effectively purifies and concentrates the hemagglutinin of the FA strain of mouse encephalomyelitis virus.
Purifies mouse encephalomyelitis virus hemagglutinin via ether extraction and elution; leaves open applicability to other viruses or settings.
It has been discovered by Nakagawa and Kodama that FA strain of mouse encephalomyclitis virus agglutinates chicken red cells at 4C when 1.5% CaCl2 solution is used as a reaction medium. The present study concerns the purification and concentration of the virus hemagglutinin by application of the properties of the virus.Therefore, purification of the virus hemagglutinin by means of adsorption to and elution from erythrocytes was attempted. From the result obtained, this procedure was an effective and simple method as in the case of GD VII virus and Influenza virus. Ether extraction of crude, also, is a simple method for removal of nonviral nitrogen. In order to yield more purified hemagglutinin of FA strain, methanol and acetone precipitation methods were employed after ether extraction of virus material and elution of virus from blood cells.The results obtained indicate that a good yield of hemagglutinin of FA strain is acquired by elution from precipitants produced by adding acetone to give a final concentration of 30% to the materials, obtained after extraction by ether and adsorption to and elution from erythrocytes, and keeping the mixture at 4C for 3 hours with occasional agitation.
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Nakagawa et al. (1955) studied Mouse encephalomyelitis virus (FA strain). Purification by ether extraction, hemagglutination, and methanol/acetone precipitation was evaluated on Purification and concentration of FA-hemagglutinin. Purification of FA strain hemagglutinin of mouse encephalomyelitis virus was achieved by removing nonviral nitrogen via ether extraction and hemagglutination-elution, though methanol and acetone precipitation resulted in poor yields.
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