The Grape Fruit Bioflavonoid Naringin Protects Against the Doxorubicin-Induced Micronuclei Formation in Mouse Bone Marrow

Doxorubicin DOX is clinically used in the management of numerous neoplastic disorders however development of life threatening cardiotoxicity and normal cytotoxicity is a major impediment in realizing its full potential in cancer therapy The effect of various doses of Naringin was investigated on the DOX induced DNA damage by micronucleus assay in mice bone marrow Administration of mice with or mg kg body weight of DOX resulted in a dose dependent rise in the frequency of micro nucleated polychromatic MPCE and norm chromatic MNCE erythrocytes accompanied by a DOX dose related decline in the polychromatic erythrocytes and norm chromatic erythrocytes PCE NCE ratio The frequencies of MPCEs and MNCEs increased with scoring time and the greatest rise in MPCE was observed at h post DOX treatment whereas a maximum increase in MNCE was recorded at h post DOX treatment The PCE NCE ratio declined continuously with scoring time with a maximum decline at h post treatment Mice administered with and mg kg body weight of Naringin orally once daily for consecutive days before DOX treatment showed a significant attrition in the DOX induced micronuclei frequency accompanied by a significant rise in the PCE NCE ratio at all scoring times when compared to DOX treatment alone The greatest protection against DOX induced genotoxicity was observed for mg kg Naringin Naringin reduced the DOX induced DNA damage as indicated by a significant decline in the micronuclei formation This which may be due to inhibition of free radicals increased antioxidant status metal chelation and restoration of topoisomerase II activity by Naringin