Key result
TLR2 and TLR4 gene polymorphisms show no association with Chagas disease or chagasic cardiomyopathy.
Why the study?
Are TLR2 and TLR4 gene polymorphisms associated with susceptibility to Chagas' disease or chagasic cardiomyopathy?
Case-Control (n=475)
Are TLR2 and TLR4 gene polymorphisms associated with susceptibility to Chagas' disease or chagasic cardiomyopathy?
TLR2 and TLR4 gene polymorphisms are rare in this Colombian population and do not appear to play a significant role in susceptibility to Chagas' disease or chagasic cardiomyopathy.
No association of TLR2/4 polymorphisms with Chagas susceptibility or cardiomyopathy; leaves open other genetic or environmental contributors in endemic populations.
The aim of this study was to test the possible implication of toll-like receptor 2 (TLR2) and TLR4 gene polymorphisms in determining the susceptibility to Chagas' disease. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 475 individuals from Colombia, 143 seropositive with chagasic cardiomyopathy, 132 seropositive asymptomatic and 200 seronegative. The TLR2 arginine to glutamine substitution at residue 753(Arg753Gln) polymorphism was absent in the groups analyzed. The TLR4 Asp299Gly and Thr399Ile polymorphisms are in linkage disequilibrium and we observed a very low frequency of these polymorphisms in our study population (2.6% and 1.8% respectively). The overall TLR2 and TLR4 alleles and genotype distribution in seronegative and seropositive were not significantly different. We compared the frequencies between asymptomatic patients and those with chagasic cardiomyopathy and we did not observe any significant differences in the distribution of alleles or genotypes. In summary, this study corroborates the low frequency of TLR2 and TLR4 polymorphisms observed in other populations and suggest that these do not play an important role in Chagas' disease. The validation of these findings in independent cohorts is needed to firmly establish a role for TLR2 and TLR4 variants in Chagas' disease.
No takes yet. Share an insight, caveat, or question.
Zafra et al. (2008) conducted a case-control in Chagas' disease (n=475). Toll-like receptor 2 (TLR2) and TLR4 gene polymorphisms vs. Absence of polymorphisms was evaluated on Susceptibility to Chagas' disease (allele and genotype distribution). Toll-like receptor 2 and 4 gene polymorphisms occurred at very low frequencies and were not significantly associated with susceptibility to Chagas' disease or chagasic cardiomyopathy.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: