Key result
Adipocyte differentiation increases mouse PDE3B gene promoter activity in 3T3-L1 cells.
Why the study?
Does adipocyte differentiation activate the PDE3B gene promoter in 3T3-L1 cells?
Does adipocyte differentiation activate the PDE3B gene promoter in 3T3-L1 cells?
The study demonstrates that adipocyte differentiation activates the mouse PDE3B gene promoter, which may have implications for understanding and improving insulin resistance.
Does not yet change practice; leaves open PDE3B promoter relevance to human insulin resistance.
Activation of phosphodiesterase (PDE) 3B reduces free fatty acid output from adipocytes. Induction of PDE3B gene expression by adipocyte differentiation could improve insulin resistance. To examine whether the PDE3B promoter is activated by this differentiation, the 5' flanking sequence of the mouse PDE3B gene was isolated. The transcription initiation site was determined to be located 195 bp upstream of the translation start site. No putative binding site for peroxisome proliferator-activated receptor gamma was found within 2 kb upstream of the transcription initiation site. This region had promoter activity, which was further activated on adipocyte differentiation in 3T3-L1 cells.
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Niiya et al. (2001) studied this question. Adipocyte differentiation was evaluated on PDE3B promoter activity. The 2 kb upstream region of the mouse PDE3B gene exhibited promoter activity that was further activated upon adipocyte differentiation in 3T3-L1 cells.
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