Key result
PMA, IL-1, TNF, thrombin, and neuropeptide Y fail to increase Endothelin-1 secretion in cultured endothelial cells.
This study demonstrates that human umbilical vein endothelial cells actively secrete Endothelin-1 in vitro.
Endothelial ET-1 secretion in culture should not change practice; leaves open in vivo regulation and disease relevance.
Endothelin-1 (ET-1) has been identified in the conditioned medium of porcine endothelial cells. Human endothelin (ET-1) cloned from a placenta cDNA library is similar to porcine, but it is not known whether endothelin itself is secreted by human endothelial cells. To answer this question, a conditioned medium taken every 48 h from confluent cultures of umbilical vein endothelial cells was analyzed by HPLC and all fractions were tested for their ability to inhibit [125I]ET-1 binding on human placenta membranes. Only one fraction did inhibit [125I]ET-1 binding. When the conditioned medium was spiked with ET-1, the same single fraction inhibited [125I]ET-1 binding showing that ET-1, itself, is present in the conditioned medium of human endothelial cells. ET-1 accumulates with time, reaching a plateau at 48 h. ET-1 secretion is not increased by a 24-h incubation of endothelial cells with phorbol myristate acetate, interleukin-1, tumor necrosis factor, thrombin or neuropeptide Y.
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Walter Fischli (1989) studied this question. Human umbilical vein endothelial cell culture was evaluated on Presence of Endothelin-1 in conditioned medium. Human cultured umbilical vein endothelial cells secrete Endothelin-1, which accumulates over 48 hours and is not increased by PMA, IL-1, TNF, thrombin, or neuropeptide Y.
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