September 1, 1991Circulation Research

Platelets stimulate expression of endothelin mRNA and endothelin biosynthesis in cultured endothelial cells.

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Population

Cultured endothelial cells

Comparison

Coincubation with washed human platelets… vs Basal levels (no coincubation)

Design

Preclinical

Authors

EOEliot H. OhlsteinHeart Failure & Transplant BSBarbara L. StorerNew Frontier JBJ A ButcherAugusta University Health

Discussion

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Implication

May link platelets to endothelial endothelin production; leaves open in vivo relevance for vasomotor control.

Structured PICO

Population

Cultured endothelial cells (bovine pulmonary artery endothelial [BPAE] cells or human umbilical vein endothelial cells)

Intervention

Coincubation with washed human platelets (approximately 200,000 cells/microliters), thrombin, transforming growth factor-beta 1, or platelet-free buffer from agonist-induced platelet aggregation

Comparator

Basal levels (no coincubation)

Outcome

Immunoreactive endothelin (irET) levels and preproendothelin-1 mRNA expressionsurrogate

Platelet-derived regulatory factors can induce endothelin biosynthesis in cultured endothelial cells, suggesting a novel intercellular interaction for vasomotor regulation.

Cite This Study

Ohlstein et al. (1991) studied this question.

synapsesocial.com/papers/6a9623471185bf4ef03d48b5 https://doi.org/10.1161/01.res.69.3.832

View Full Paper

Ask AI

Bookmark

Platelets stimulate expression of endothelin mRNA and endothelin biosynthesis in cultured endothelial cells.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Platelets stimulate expression of endothelin mRNA and endothelin biosynthesis in cultured endothelial cells.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider