Key result
In vitro lipase inhibitors blunt heparin-induced NEFA elevations, suggesting drug binding changes are laboratory artifacts.
Why the study?
Does the in vitro addition of lipoprotein lipase inhibitors reduce heparin-induced alterations in protein drug binding in blood samples from healthy subjects?
Population
11 healthy subjects
Comparison
Intravenous heparin with subsequent in vitro… vs Blood samples before heparin administration and…
Design
Other
Follow-up
15 minutes
Authors
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May not alter in vivo drug exposure; leaves open clinical relevance pending in vivo confirmation.
Does the in vitro addition of lipoprotein lipase inhibitors reduce heparin-induced alterations in protein drug binding in blood samples from healthy subjects?
p-value: p=<0.001
Heparin-induced changes in protein drug binding are largely in vitro artifacts caused by continued triglyceride lipase activity.
Brown et al. (1981) studied Healthy subjects (n=11). Intravenous heparin and in vitro lipase inhibitors (protamine and EDTA) vs. Blood samples before heparin and without lipase inhibitors was evaluated on Change in nonesterified fatty acid (NEFA) concentration and free fractions of lidocaine, diazepam, and propranolol (p=<0.001). In vitro addition of lipase inhibitors diminished heparin-induced elevations of NEFA and free fractions of lidocaine and diazepam (P<0.001), suggesting these binding changes are largely in vitro artifacts.
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