Key result
Over a 4-hour period, human lipoprotein lipase and human hepatic lipase significantly reduced the platelet macroaggregatory response to collagen from 17.0 Ohms to 1.6 and 1.2 Ohms, respectively.
Why the study?
Does the in vitro addition of heparin-releasable lipolytic enzymes reduce platelet macroaggregation in whole blood from healthy volunteers?
Population
Blood samples from 34 healthy volunteers who had not taken non-steroidal anti-inflammatory drugs or other…
Design
Preclinical
Follow-up
4 hours (in vitro)
Authors
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Suggests mechanism for platelet dysfunction in cardiopulmonary bypass; hypothesis-generating and requires clinical confirmation.
Does the in vitro addition of heparin-releasable lipolytic enzymes reduce platelet macroaggregation in whole blood from healthy volunteers?
Absolute Event Rate: 1.6% vs 17%
p-value: p=<0.01
Heparin-induced lipolysis generates lipoprotein degradation products that inhibit platelet macroaggregation, providing a potential mechanism for the platelet dysfunction observed during cardiopulmonary bypass.
Muriithi et al. (2002) studied Healthy volunteers (n=34). Human lipoprotein lipase and human hepatic lipase vs. Baseline (time 0) was evaluated on Platelet macroaggregatory response to ex vivo stimulation with collagen (0.6 µg ml-1) at 4 hours (p=<0.01). Over a 4-hour period, human lipoprotein lipase and human hepatic lipase significantly reduced the platelet macroaggregatory response to collagen from 17.0 Ohms to 1.6 and 1.2 Ohms, respectively.
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