Key result
AMP and IMP cause actin liberation and reversible actomyosin dissociation, contributing to rigor mortis resolution.
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AMP and IMP are identified as key factors causing actin liberation and reversible dissociation of actomyosin, potentially explaining the resolution of rigor mortis in skeletal muscles.
We investigated to determine why heating of squid muscle at 60 degrees C induced the liberation of actin from myofibrils. When a mixture of a myofibrillar fraction and a low-molecular sarcoplasmic fraction prepared from squid muscle was heated at 60 degrees C, actin liberation occurred. When a myofibrillar fraction was heated with ATP, AMP, or IMP, actin liberation occurred. Hence, AMP is perhaps one of the factors causing actin liberation in postmortem squid muscle. It was found that AMP and IMP reversibly dissociated actomyosin of chicken, bovine, and porcine skeletal muscles into actin and myosin on incubation at 0 degrees C at pH 7.2 in 0.2 M KCl. These results led us to conclude that AMP and IMP were the most responsible factors causing actin liberation from myofibrils in the heated muscle and causing reversible dissociation of actomyosin on storage of skeletal muscle at a low temperature. Hence, AMP and IMP are possible factors causing the resolution of rigor mortis in muscles.
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OKITANI et al. (2008) studied this question. AMP and IMP was evaluated on Actin liberation from myofibrils and reversible dissociation of actomyosin. AMP and IMP caused actin liberation from myofibrils in heated muscle and reversible dissociation of actomyosin during low-temperature storage, suggesting they contribute to rigor mortis resolution.
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