Key result
Inosine-5'-monophosphate solubilized actomyosin and myofibrils in the presence of pyrophosphate or ADP, suggesting it is a candidate agent to resolve rigor mortis.
IMP, in combination with pyrophosphate or ADP, can dissociate actomyosin and is a candidate agent to resolve rigor mortis in skeletal muscle.
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May aid rigor mortis resolution in skeletal muscle models; leaves open human translation and clinical utility.
The object of the present study was to reveal the action of inosine-5'-monophosphate (IMP) toward myofibrils in postmortem muscles. IMP solubilized isolated actomyosin within a narrow range of KCl concentration, 0.19-0.20 mol/L, because of the dissociation of actomyosin into actin and myosin, but it did not solubilize the proteins in myofibrils with 0.2 mol/L KCl. However, IMP could solubilize both proteins in myofibrils with 0.2 mol/L KCl in the presence of 1 m mol/L pyrophosphate or 1.0-3.3 m mol/L adenosine-5'-diphosphate (ADP). Thus, we presumed that pyrophosphate and ADP released thin filaments composed of actin, and thick filaments composed of myosin from restraints of myofibrils, and then both filaments were solubilized through the IMP-induced dissociation of actomyosin. Thus, we concluded that IMP is a candidate agent to resolve rigor mortis because of its ability to break the association between thick and thin filaments.
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MATSUISHI et al. (2016) studied Rigor mortis of skeletal muscle. Inosine-5'-monophosphate (IMP) was evaluated on Solubilization of isolated actomyosin and myofibrils. Inosine-5'-monophosphate solubilized actomyosin and myofibrils in the presence of pyrophosphate or ADP, suggesting it is a candidate agent to resolve rigor mortis.
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