Key result
Unstimulated human PMNs markedly inhibit platelet aggregation and ATP release.
Why the study?
The effect of unstimulated human polymorphonuclear leukocytes on platelet activation and the mechanisms involved were not fully understood.
Unstimulated human polymorphonuclear leukocytes inhibit platelet aggregation and ATP release through an uncharacterized mechanism independent of arachidonic acid metabolites, oxygen reactive intermediates, nitric oxide, or proteases.
Hypothesis-generating for PMN-mediated platelet inhibition; leaves open mechanism and in vivo relevance.
The effect of unstimulated human polymorphonuclear leukocytes (PMNs) on platelet activation was examined. 2. Human platelet aggregation and adenosine 5'-triphosphate (ATP) release induced by collagen (1-2 micrograms ml-1); thrombin (0.01-0.02 u ml-1) or arachidonic acid (AA) (0.1-0.2 mM) were markedly inhibited when conducted in the presence of unstimulated PMNs. 3. Platelet inhibition induced by PMNs was dependent on the number of PMNs and on the incubation time of the mixed cell suspension. 4. Platelet inhibition was not reversed in time when PMNs were depleted from the mixed-cell suspension. 5. PMN-mediated platelet-inhibition was not mediated by AA metabolites, oxygen reactive intermediates, nitric oxide or proteases. 6. The factor(s) accounting for the platelet inhibition mediated by PMNs are not yet characterized.
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Schattner et al. (1990) studied this question. Unstimulated human polymorphonuclear leukocytes (PMNs) was evaluated on Platelet aggregation and ATP release. Unstimulated human polymorphonuclear leukocytes markedly inhibited platelet aggregation and ATP release induced by collagen, thrombin, or arachidonic acid.
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