Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
September 14, 2026International MicrobiologyOpen Access

Comparative genomics reveals genotype-phenotype concordance and cryptic resistomes in clinical Pseudomonas aeruginosa

View Full Paper
Ask AI
Bookmark
Share

Authors

SRSweta Padma RoutrayTSTripti SwarnkarESEnketeswara Subudhi

Discussion

Loading...

Member takes

Overview

Comparative genomic analysis reveals cryptic resistomes and phenotypic concordance in clinical Pseudomonas aeruginosa, highlighting the limitations of phenotype-only diagnostics.

Key Points

  • To evaluate antimicrobial resistance architecture and genotype-phenotype concordance in clinical Pseudomonas aeruginosa isolates using combined phenotypic profiling and whole-genome sequencing.
  • Selected representative multidrug-resistant (MDR) and non-MDR isolates from 1,295 culture-positive clinical specimens collected at a tertiary care hospital in eastern India.
  • Assessed antimicrobial susceptibility via VITEK-2 and Kirby-Bauer disk diffusion, and confirmed species identity using 16S rRNA sequencing.
  • Conducted reference-guided whole-genome sequencing to profile resistance determinants using the CARD database and built phylogenetic trees against 454 reference genomes.
  • MDR isolates exhibited greater sequence divergence relative to the PA14 reference (~69,000 variants) than the non-MDR isolate (~58,700 variants), with >92% coverage at ≥30X depth.
  • MDR isolates showed strong genotype-phenotype concordance across five antibiotic classes, linked to specific β-lactamase variants (PDC-67, OXA-396) and regulatory adaptations (ArmR, cprS).
  • The non-MDR isolate carried silent resistance determinants—including gyrA (T83I), PDC-1, and OXA-847—without expressing resistance phenotypes, and mapped to a distinct phylogenetic lineage.

Cite This Study

Routray et al. (2026) studied this question.

synapsesocial.com/papers/6aa7b2e10926e14a848b1748https://doi.org/10.1007/s10123-026-00892-3
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Integrated FTIR and Whole-Genome Sequencing Reveal Scale-Dependent Genotype–Phenotype Relationships in Multidrug-Resistant Pseudomonas aeruginosa2026
  2. 2High-Risk Clones of Carbapenem-Resistant Pseudomonas aeruginosa in India: Genome and Traits2026
  3. 3Unveiling the microevolution of antimicrobial resistance in selected Pseudomonas aeruginosa isolates from Egyptian healthcare settings: A genomic approach2024 · 10 citations
  4. 4Genomic Analysis of Antimicrobial Resistance in Pseudomonas aeruginosa from a “One Health” Perspective2024 · 6 citations
  5. 5Phenotypic & genotypic profile of antimicrobial resistance in Pseudomonas species in hospitalized patients2019 · 15 citations