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September 15, 2026Microbiology

Preservation of Strictly Acidophilic Autotrophic Bacteria Acidithiobacillus ferrooxidans by Lyophilization Using Steel

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Authors

AYAlyona YachkulaAZAnton ZvonarevEKE. B. Kudryashova

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Overview

Experimental study reveals enhanced post-rehydration viability of Acidithiobacillus ferrooxidans freeze-dried with steel wire, indicating improved long-term storage for biomining bacteria.

Key Points

  • Evaluate whether supplementing a sucrose-gelatin-agar protector medium with steel wire improves cell survival of strictly acidophilic Acidithiobacillus ferrooxidans during lyophilization and rehydration.
  • Lyophilized two strains of Acidithiobacillus ferrooxidans using sucrose + gelatin + agar (SGA) alone or SGA supplemented with steel wire (SGA + steel).
  • Assessed bacterial viability and post-rehydration growth dynamics using fluorescence microscopy.
  • Post-rehydration cell viability reached 63–68% with SGA + steel wire compared to 32–33% with SGA alone.
  • Microscopic analysis revealed abundant post-rehydration bacterial proliferation and biofilm formation along the steel wire surface.

Cite This Study

Yachkula et al. (2026) studied this question.

synapsesocial.com/papers/6aa913609013453be30a126ehttps://doi.org/10.1134/s0026261726600990
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