Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis

Figures

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  Fig. 1. An overview of Reads Distribution and Chromosome Distribution of RNA sequencing. a Reads number onto the regions of CDS, exons, intergenic region (InterGenic), introns, transcription end site (TES), transcription start site (TSS), 3’-UTR and 5’-UTR. b Distribution of reads on chromosomes

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  Fig. 2. Clustering of differentially expressed genes. a PCA cluster analysis of these 6 samples. b Gene thermal map of differentially expressed genes

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  Fig. 3. Gene Ontology (GO) Analysis of differentially expressed genes. GO analysis was annotated from three levels: BP, MF and CC. -Log10(P-value) was showed at abscissa axis and GO terms was showed at longitudinal axis. P value < 0.05 for all significant GO terms. BP: Biological Process; CC: Cellular Component; MF: Molecular Function

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  Fig. 4. Pathway enrichment analysis of differentially expressed genes based on KEGG. a Histogram of pathway enrichment. -Log10(P-value) was showed at abscissa axis and pathway terms of KEGG was showed at longitudinal axis. b Top 20 of pathway enrichment was displayed by different colors and different sizes of the circle

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  Fig. 5. Gene Act network analysis. Red circles represent up-regulated genes in hypertension group; Green circles represent down-regulated genes in hypertension group, arrows indicate the direction of regulation

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  Fig. 6. Western blotting validation of relative expression levels of representative differentially expressed genes. a Western blotting of 10 representative differentially expressed genes from 4 hypertensive (H1, H2 H3 and H4) and 4 normotensive (N1, N2, N3, N4) military pilots. b Statistics of relative expression of Western blotting. Bars = means ± SD. *P < 0.05, **P < 0.01, NS, not significant, n = 4