What is the clinical evidence from this study?

Intervention: HMEC-1 extracellular matrix vs. HUVEC extracellular matrix. Primary outcome: Thrombus formation and platelet aggregation.

What does this research mean for the field?

Platelet adhesion and thrombus formation can occur at high shear rates on microvascular endothelial subendothelium in the absence of von Willebrand factor and collagen types I, III, and VI. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

January 1, 2001Thrombosis and HaemostasisOpen Access

The Subendothelium of the HMEC-1 Cell Line Supports Thrombus Formation in the Absence of von Willebrand Factor and Collagen Types I, III and VI

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Key result

HMEC-1 subendothelium supports platelet adhesion and aggregation at high shear rates without collagen or vWF.

Structured PICO

Population

Human microvascular endothelial cell line (HMEC-1) and human umbilical vein endothelial cells (HUVEC) extracellular matrix models, perfused with low-molecular-weight heparin-anticoagulated human blood (including blood from a patient with severe type III von Willebrand disease).

Exposure

Blood perfusion over HMEC-1 extracellular matrix (which lacks collagen types I, III, VI and vWF) at moderate (650/s) and high (2,600/s) arterial wall shear rates.

Comparator

Blood perfusion over HUVEC extracellular matrix (which contains collagen types I, III, VI and vWF).

Outcome

Platelet adhesion and aggregate/thrombus formation.surrogate

Platelet adhesion and large thrombus formation can occur at high shear rates on microvascular subendothelium without the participation of von Willebrand factor and fibrillar collagens.

Discussion

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Implication

Challenges vWF-collagen dependence in high-shear adhesion; hypothesis-generating for microvascular thrombosis mechanisms, pending human studies.

Cite This Study

Bonnefoy et al. (2001) studied this question. HMEC-1 extracellular matrix vs. HUVEC extracellular matrix was evaluated on Thrombus formation and platelet aggregation. Platelet adhesion and large aggregate formation on HMEC-1 subendothelium occurred at high shear rates without the participation of collagen types I, III, and VI, and von Willebrand factor.

synapsesocial.com/papers/6aa9591aa35b18e46c05013f https://doi.org/10.1055/s-0037-1615620

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Implication

Challenges vWF-collagen dependence in high-shear adhesion; hypothesis-generating for microvascular thrombosis mechanisms, pending human studies.

The Subendothelium of the HMEC-1 Cell Line Supports Thrombus Formation in the Absence of von Willebrand Factor and Collagen Types I, III and VI

Structured PICO

Discussion

Implication

Cite This Study

The Subendothelium of the HMEC-1 Cell Line Supports Thrombus Formation in the Absence of von Willebrand Factor and Collagen Types I, III and VI

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider