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A rapid and precise RP-HPLC method has been developed for the validated of Zinostatin in its pure form as well as in tablet dosage form.Chromatography was carried out on Apollo C18 (4.6×150mm, 5µ) column using a mixture of Methanol and water (80:20 v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 232nm.The retention time of the Zinostatin was 3.0 ±0.02min.The method produce linear responses in the concentration range of 25-115µg/ml of Zinostatin.The method precision for the determination of assay was below 2.0%RSD.The method is useful in the quality control of bulk and pharmaceutical formulations.
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Farheen et al. (2019) studied this question.
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