Key result
Targeted siRNA cocktails reduce BVDV-1 antigen production up to ~40% in vitro.
Why the study?
Bovine viral diarrhea viruses are important cattle pathogens worldwide with no available antiviral therapy.
siRNAs targeting the 5'-UTR and structural envelope protein genes can effectively inhibit BVDV-1 replication in vitro.
In vitro siRNA activity against BVDV-1 is hypothesis-generating; leaves open translation to animal models or clinical use.
Bovine viral diarrhea viruses (BVDVs) are important pathogens of cattle that occur worldwide, and for which no antiviral therapy is available. In the present study, the inhibitory effect of small interfering (si) RNAs on bovine viral diarrhea virus 1 (BVDV-1) replication in cultured bovine cells was explored. Four synthetic siRNAs were designed to target structural envelope region genes (Erns, E1, and E2) and one cocktail of siRNA was generated to target the 5ʹ-UTR of the BVDV-1 genome. The inhibitory effects of siRNAs were assessed by determination of infectious viral titer, viral antigen and viral RNA. The siRNA cocktail and three of the synthetic siRNAs produced moderate anti-BVDV-1 effect in vitro as shown by 25%-40% reduction in BVDV-1 antigen production, 7.9-19.9-fold reduction in viral titer and 21-48-fold reduction in BVDV-1 RNA copy number. Our findings suggest that siRNA cocktail targeted at the 5ʹ-UTR is a stronger inhibitor of BVDV-1 replication and the targets for siRNA inhibition can be extended to BVDV-1 structural envelope protein genes.
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Mishra et al. (2011) studied Bovine viral diarrhea virus 1 (BVDV-1) infection. siRNA cocktail and synthetic siRNAs vs. Negative control siRNA was evaluated on BVDV-1 replication (viral antigen, titer, and RNA copy number). A siRNA cocktail targeting the 5'-UTR and synthetic siRNAs targeting structural envelope genes reduced BVDV-1 antigen production by 25% to 40% and viral RNA copy number by 21- to 48-fold in vitro.
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