Key result
Ex vivo FITC labeling below 0.55 mg/10^11 platelets preserves platelet viability and physiological function.
Why the study?
The effects of ex vivo fluorescent labeling of blood platelets on platelet viability and function were not well characterized.
FITC labeling of platelets does not impair their physiological properties at concentrations below 0.55 mg/10^11 platelets, making it suitable for vital microscopic observations.
Supports optimized ex vivo platelet labeling; leaves open in vivo and clinical translation.
Possible effects of an ex vivo fluorescent labeling procedure of blood platelets on the platelet viability were examined by different function tests. The degree of the impairment of the platelet function proved to be principally correlated with the dye concentration used during the labeling procedure. However, the physiological properties of the labeled platelets obviously remain unrestricted by using intraplatelet fluorescein isothiocyanate (FITC) concentrations below 0.55 mg/10(11) platelets, corresponding to 8.5 X 10(6) FITC molecules bound to one platelet. A procedure for fluorescent labeling of blood platelets is described which is considered to be preferably suitable to prepare platelets for vital microscopic platelet observations.
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Klaverkamp et al. (2009) studied this question. Fluorescein isothiocyanate (FITC) labeling was evaluated on Platelet viability and function. Ex vivo fluorescent labeling of blood platelets with FITC concentrations below 0.55 mg/10(11) platelets maintains platelet viability and physiological properties.
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