Key result
Oral quinapril cuts mononuclear leukocyte reactive oxygen species generation by ~50%.
Why the study?
Angiotensin-converting enzyme inhibitors may affect reactive oxygen species production and NAD(P)H oxidase activity in humans in vitro and in vivo.
Do ACE inhibitors reduce reactive oxygen species generation in mononuclear leukocytes?
Do ACE inhibitors reduce reactive oxygen species generation in mononuclear leukocytes?
Absolute Event Rate: 988% vs 1981%
p-value: p=<0.01
Quinapril and captopril decrease the production of reactive oxygen species and inhibit NAD(P)H oxidase activity in mononuclear leukocytes.
May reduce leukocyte ROS in experimental models; leaves open human cardiovascular translation.
BACKGROUND: Angiotensin-converting enzyme inhibitors may affect reactive oxygen species in humans in vitro and in vivo. In the present study we evaluated whether angiotensin-converting enzyme inhibitors may affect NAD(P)H oxidase activity. MATERIALS AND METHODS: The production of reactive oxygen species was measured spectrophotometrically in mononuclear leukocytes using the fluorescent dye, dichlorofluorescein diacetate. The effects of quinaprilat, captopril, enalaprilat and lisinopril on phorbol myristate acetate-induced reactive oxygen species generation were investigated in vitro. The effects of quinaprilat, captopril, enalaprilat and lisinopril on the NAD(P)H oxidase activity of the mononuclear leukocytes were measured photometrically. In addition, reactive oxygen species were measured before and 4 h after oral administration of quinapril. RESULTS: In vitro, the addition of quinaprilat (72 +/- 6% of control; mean +/- SEM; n= 19; P < 0.001) and captopril (48 +/- 2% of control; n= 19; P < 0.001) significantly reduced the phorbol-12-myristate-13-acetate-induced reactive oxygen species generation by the mononuclear leukocytes, whereas enalaprilat and lisinopril showed no effect. The effect of captopril on phorbol-12-myristate-13-acetate-induced reactive oxygen species generation in vitro was concentration-dependent. Quinaprilat and captopril significantly inhibited the NAD(P)H oxidase activity. After the oral administration of 10 mg of quinapril the phorbol-12-myristate-13-acetate-induced reactive oxygen species generation by the mononuclear leukocytes was significantly decreased from 1981 +/- 292% to 988 +/- 141% (n = 14; P < 0.01). CONCLUSION: Quinapril and captopril decrease the production of reactive oxygen species.
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Giet et al. (2002) studied this question. Quinapril and captopril vs. Baseline (in vivo) and control (in vitro) was evaluated on Phorbol-12-myristate-13-acetate-induced reactive oxygen species generation (p=<0.01). Oral administration of 10 mg quinapril significantly decreased reactive oxygen species generation by mononuclear leukocytes from 1981% to 988% (P<0.01).
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