What is the clinical evidence from this study?

Study design: Cross-Sectional. Population: Gastrointestinal infection (n=250). Intervention: Multiplex real-time quantitative PCR assay for HBoV1-4. Primary outcome: Detection of HBoV1-4.

What does this research mean for the field?

A novel multiplex real-time PCR assay reliably detects human bocaviruses 1 through 4, identifying HBoV2 in 1.6% and HBoV3 in 0.4% of patients evaluated for gastrointestinal infection. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.SUPPORTS_CONSENSUS.

September 15, 2010Journal of Clinical MicrobiologyOpen Access

Real-Time Quantitative PCR Detection of Four Human Bocaviruses

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Key result

Novel multiplex PCR assay detects HBoV2 in 1.6% of stool samples evaluated for gastrointestinal infection.

n=250

Population

250 Finnish patients (median age, 40 years) with gastrointestinal infection (stool samples)

Design

Cross-sectional

Authors

KKKalle Kantola MSMohammadreza Sadeghi JAJenni Antikainen

Discussion

Member takes

Overview

Supports HBoV2 detection in GI evaluations; hypothesis-generating for its role in pediatric diarrhea and needs controlled studies.

Study Design

Type

Cross-Sectional (n=250)

Structured PICO

Population

250 Finnish patients (median age 40 years) whose stool samples were sent for diagnosis of gastrointestinal infection.

Exposure

Multiplex real-time quantitative PCR assay for detection of human bocaviruses (HBoV1-4)

Outcome

Detection and quantification of HBoV1-4 DNAsurrogate

A novel multiplex real-time PCR assay successfully detects and quantifies four human bocaviruses, identifying HBoV2 as a relatively common pathogen in young children with diarrhea.

Cite This Study

Kantola et al. (2010) conducted a cross-sectional in Gastrointestinal infection (n=250). Multiplex real-time quantitative PCR assay for HBoV1-4 was evaluated on Detection of HBoV1-4. A novel multiplex real-time PCR assay detected HBoV2 in 1.6% and HBoV3 in 0.4% of 250 stool samples from Finnish patients evaluated for gastrointestinal infection.

synapsesocial.com/papers/6aa9f89533211e1322fc9501 https://doi.org/10.1128/jcm.00686-10

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September 15, 2010Journal of Clinical MicrobiologyOpen Access

Real-Time Quantitative PCR Detection of Four Human Bocaviruses

View Full Paper

Ask AI

Bookmark

Key result

Novel multiplex PCR assay detects HBoV2 in 1.6% of stool samples evaluated for gastrointestinal infection.

n=250

Population

250 Finnish patients (median age, 40 years) with gastrointestinal infection (stool samples)

Design

Cross-sectional

Authors

KKKalle Kantola MSMohammadreza Sadeghi JAJenni Antikainen

Discussion

Member takes

Overview

Supports HBoV2 detection in GI evaluations; hypothesis-generating for its role in pediatric diarrhea and needs controlled studies.

Study Design

Type

Cross-Sectional (n=250)

Structured PICO

Population

250 Finnish patients (median age 40 years) whose stool samples were sent for diagnosis of gastrointestinal infection.

Exposure

Multiplex real-time quantitative PCR assay for detection of human bocaviruses (HBoV1-4)

Outcome

Detection and quantification of HBoV1-4 DNAsurrogate

A novel multiplex real-time PCR assay successfully detects and quantifies four human bocaviruses, identifying HBoV2 as a relatively common pathogen in young children with diarrhea.

Cite This Study

synapsesocial.com/papers/6aa9f89533211e1322fc9501 https://doi.org/10.1128/jcm.00686-10

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