Key result
CVB3-induced acute viral myocarditis increases splenic CD80+ B cells by ~78%, correlating with disease severity.
Why the study?
Viral myocarditis pathogenesis is unclear but involves excessive immune responses, and whether CD80+/CD86+ B cells participate in acute viral myocarditis pathogenesis was unknown.
CD80+ B cells are significantly increased and correlate with disease severity in a mouse model of CVB3-induced acute myocarditis, suggesting a role in its pathogenesis.
May implicate CD80+ B cells in murine CVB3 myocarditis pathogenesis; leaves open relevance to human disease.
INTRODUCTION: The pathogenesis of viral myocarditis (VMC) is unclear, but many studies have shown that VMC is associated with an excessive immune response. CD80 and CD86 are important costimulatory molecules that play a critical role in autoimmunity. However, whether CD80+/CD86+ B cells participate in the pathogenesis of acute VMC is unknown. MATERIAL AND METHODS: Male C57BL/6 mice were infected by intraperitoneal injection with coxsackievirus B3 (CVB3) to establish a VMC model. Control mice were administered phosphate-buffered saline intraperitoneally. At one week and two weeks post injection, histopathological changes in heart tissue were assessed with haematoxylin and eosin staining. The frequency of splenic CD80+/CD86+ B cells was measured with flow cytometry. RESULTS: The frequency of CD80+ B cells was significantly increased in VMC, while the frequency of CD86+ B cells was significantly decreased. Furthermore, the frequency of CD80+ B cells related to the severity of VMC. CONCLUSIONS: These data show that CD80+/CD86+B cells are involved in the pathogenesis of VMC, with CD80+B cells being more important than CD86+B cells.
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Huang et al. (2020) studied Viral myocarditis (n=36). Coxsackievirus B3 (CVB3) infection vs. Phosphate-buffered saline (PBS) was evaluated on Frequency of splenic CD80+ B cells at 1 week (p=<0.01). Coxsackievirus B3-induced acute viral myocarditis significantly increased the frequency of splenic CD80+ B cells compared to controls (7.62% vs 4.29%, p<0.01), which correlated with disease severity.
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