Key result
Active Na transport entirely drives active proton extrusion in rabbit proximal tubules lacking bicarbonate.
Why the study?
The mechanisms of H+/OH- transport and the presence of a proton pump in the rabbit proximal convoluted tubule in the absence of bicarbonate were unclear.
Population
Rabbit proximal convoluted tubule perfused in vitro
Comparison
Blockade of apical Na/H exchange, bilateral Na removal, and removal of luminal glucose and alanine versus control conditions
Design
Preclinical in vitro study
Authors
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Should not change clinical acid-base management; hypothesis-generating for Na-dependent proton extrusion in mammalian proximal tubule.
In the absence of bicarbonate, all active proton extrusion in the rabbit proximal convoluted tubule is dependent on active Na transport, with negligible proton leak.
Beck et al. (1993) studied this question. Blockade of apical Na/H exchange and bilateral Na removal vs. Baseline control was evaluated on Intracellular pH (pHi) and membrane potential. In the absence of bicarbonate, all active proton extrusion in the rabbit proximal convoluted tubule is dependent on active Na transport, with negligible proton leak.
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