Aligners are the most important software used in the field of Transcriptomics studies and related fields. In the recent study, almost all the aligners could be configured to give good results, but still, researchers and scientists who use such software face challenges in choosing accurate, sensitive, requiring fewer hardware facilities and ultimately appropriate with their research goals. We try to clarify the various challenges and misunderstandings, below.
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Bahrami Abolfazl (2020) studied this question.
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