Key result
New HPLC method quantifies diltiazem in human cardiac tissue with ~59% recovery.
Why the study?
A sensitive and reliable method for quantifying diltiazem in human cardiac tissue was needed to support pharmacokinetic and therapeutic studies.
A new HPLC method was successfully developed and validated for the quantitation of diltiazem in human cardiac tissue.
Enables diltiazem cardiac tissue quantification in research; leaves open any clinical translation or dosing implications.
A new high-performance liquid chromatographic method has been developed for the determination of diltiazem in human cardiac tissue. Tissue samples are homogenized and digested with trypsin solution. Diltiazem and the internal standard are extracted with acetone. The extract is evaporated to dryness and reconstituted in potassium phosphate buffer. Samples are then cleaned up with solid-phase extraction columns. Diltiazem and the internal standard show recoveries of 59% +/- 16 and 52% +/- 13. The linearity range is 0.12-2.25 ng/mg wet weight. The limit of quantitation is 0.12 ng/mg (w/w). The percentage coefficient of variation of intra-assay varies between 3.57 and 11.2, and that of interassay varies between 5.42 and 11.7. As an application of the assay, a diltiazem cardiac tissue level in a patient on oral therapy for supraventricular tachycardia is reported.
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Läer et al. (1997) studied Supraventricular tachycardia (n=1). High-performance liquid chromatography (HPLC) method was evaluated on Diltiazem recovery, linearity range, and limit of quantitation. A new HPLC method for quantifying diltiazem in human cardiac tissue demonstrated recoveries of 59% +/- 16, a linearity range of 0.12-2.25 ng/mg, and a limit of quantitation of 0.12 ng/mg.
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