Key result
Automated PatchXpress matches conventional hERG inhibition data with higher throughput and ~65% success rates.
Why the study?
An assay to evaluate hERG potassium channel inhibition with higher throughput and comparable accuracy to conventional patch clamp methods was needed.
Population
Monoclonal cell line stably expressing hERG potassium channels in CHO-KI cells
Comparison
PatchXpress automated 16-channel parallel patch clamp system vs conventional patch clamp experiments
Design
Preclinical assay development study
Authors
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May facilitate higher-throughput hERG assays; leaves open translation beyond animal cells.
The PatchXpress automated system provides a high-throughput assay for evaluating hERG channel inhibition that closely matches data produced using conventional patch clamp methods.
Ly et al. (2005) studied this question. PatchXpress automated patch clamp system vs. Conventional patch clamp experiments was evaluated on Success rates for cell recordings. The PatchXpress automated patch clamp system achieved success rates up to 55-65% using Accumax treatment and produced hERG inhibition data closely matching conventional methods with greater throughput.
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